Inhibiting roles of FOXA2 in liver cancer cell migration and invasion by transcriptionally suppressing microRNA-103a-3p and activating the GREM2/LATS2/YAP axis

Cytotechnology - Forkhead box A2 (FOXA2) has emerged as a tumor inhibitor in several human malignancies. This work focused on the effect of FOXA2 on liver cancer (LC) cell invasion and migration...     

小麦倒春寒研究现状与进展

由于全球气候变暖,近年来小麦低温灾害事件频发,尤其是拔节-孕穗期的倒春寒灾害已成为制约小麦产量和品质的重要因素之一。本文综述了小麦倒春寒灾害的发生特点(鉴定与分级、时空特征),倒春寒对小麦生理特性(叶片、茎秆、穗部、根系)和产量、质量的影响,总结了抗倒春寒小麦育种、倒春寒危害的分子生物学机制及灾害的监测预警与风险评估等方面的研究进展,并从小麦抗倒春寒遗传基础、倒春寒危害小麦评价体系和防控技术体系等方面进行了展望,以期为抗倒春寒小麦品种的遗传改良和栽培调控新措施的建立提供理论依据。     

Blocking of the PD-1/PD-L1 interaction by a novel cyclic peptide inhibitor for cancer immunotherapy

The interaction of PD-1/PD-L1 allows tumor cells to escape from immune surveillance. Clinical success of the antibody drugs has proven that blockade of PD-1/PD-L1 pathway is a promising strategy for cancer immunotherapy. Here, we developed a cyclic peptide C8 by using Ph.D.-C7 C phage display technology. C8 showed high binding affinity with h PD-1 and could effectively interfere the interaction of PD-1/PD-L1. Furthermore, C8 could stimulate CD8+T cell activation in human peripheral blood mononuclear cells(PBMCs). We also observed that C8 could suppress tumor growth in CT26 and B16-OVA, as well as anti-PD-1 antibody resistant B16 mouse model. CD8+T cells infiltration significantly increased in tumor microenvironment, and IFN-γ secretion by CD8+T cells in draining lymph nodes also increased. Simultaneously, we exploited T cells depletion models and confirmed that C8 exerted anti-tumor effects via activating CD8+T cells dependent manner. The interaction model of C8 with h PD-1 was simulated and confirmed by alanine scanning. In conclusion, C8 shows anti-tumor capability by blockade of PD-1/PD-L1 interaction, and C8 may provide an alternative candidate for cancer immunotherapy.     

福建省直播稻根结线虫病的病原种类鉴定

[目的] 福建省福清市江镜镇与福安市潭溪镇水稻区直播稻苗期分别发生严重的根结线虫病,本研究对其病原物进行形态和分子鉴定,明确病原物种类,以期为福建省直播稻根结线虫病害防控提供理论依据。[方法] 通过根结线虫各虫态形态学特征进行观测;同时对其rDNA-ITS区进行测序,通过贝叶斯法与最大似然法构建了系统发育树来确定种类;利用拟禾本科根结线虫特异性引物Mg-F/Mg-R检测种群。[结果] 根结线虫的雌虫、雄虫和2龄幼虫的形态学特征与拟禾本科根结线虫原始描述种一致;rDNA-ITS区序列长度为576 bp,与GenBank拟禾本科根结线虫种群相似度均达99%以上;系统发育树明确了该根结线虫与拟禾本科根结线虫处于同一分支;特异性引物Mg-F/Mg-R检测进一步明确病原为拟禾本科根结线虫单一种群。[结论] 本研究通过形态与分子特征,明确了在福建直播稻上发现的根结线虫为拟禾本科根结线虫。拟禾本科根结线虫在福建省最早于2011年在政和县小范围水稻田发现,此后未在其他水稻种植区发现。本次在福建直播稻上首次发现大面积的根结线虫为害。随着直播稻的种植面积扩大,拟禾本根结线虫引起的水稻病害可能会成为制约其发展的新问题,应引起足够重视。     

小麦族植物的分类现状及主要存在的问题

小麦族(Triticeae)是禾本科、早熟禾亚科中一个有重要经济价值、以多年生植物占优势的族,族内绝大多数种类是重要的粮食作物和畜牧业上的优良牧草,饲用价值极高,有些种类具有耐寒、耐旱、耐碱等特性,是农牧业上良种繁育、牧草利用的重要基因资源。但该族同时又是分类学上的一个疑难族,各学者对族内系统分类意见不一、争议颇大,尤其在族的界限、族下类群划分以及类群演化关系上问题较多,至今尚未解决。查阅了国内外分类学文献,探讨其分类差异以及存在问题,为充分开发利用中国丰富的小麦族植物资源提供理论依据。     

Ultrastructural study of metamorphosis in the freshwater bryozoan Plumatella fungosa (Bryozoa,Phylactolaemata)

Summary

At metamorphosis the attachment of the Plumatella larva to the substrate is effected by secretions from glandular cells in the apical plate, the leading pole during swimming. The larval mantle folds back and slides down towards the substrate. By ciliary activity an adhesive secretion is spread over the metamorphosing larva and the attachment area. Two polypides appear through the larval terminal opening. The mantle fold, together with gland cells, nerve cells, sensory cells, and muscle cells from the larva form a nutritive cell mass. Reduction of this nutritive cell mass is accomplished by autolysis and phagocytosis. An invaginated area of the nutritive cell mass is provided with a dense layer of microvilli, which seem to have an absorbtive function. The nutritive cell mass consisting of transitory larval tissues provides a significant source of nutrient for the developing polypide buds.     

上升流和水团对浙江中部近海浮游动物生态类群分布的影响

依据2010年4月、7月和11月对浙江中部近海上升流海域进行的海洋调查资料,运用定量、定性方法,探讨了上升流对该海域浮游动物生态类群分布的影响.结果表明:3个季节共鉴定浮游动物64种,桡足类占主要优势,包括5个生态类群,分别是暖温带近海种、暖温带外海种、亚热带近海种、亚热带外海种和热带大洋种.在种类数组成上,春季以暖温带近海种为主,夏季则是亚热带近海种和亚热带外海种居多,秋季也是亚热带种居多,其中夏季暖温带种种类数要高于春季和秋季,这一现象与同时期东黄海沿海有所不同,主要是由于上升流将一些在海洋底部度夏的暖温种带至海洋表面造成的.此外,3个季节生态类群都是以近海种为主,表明沿岸流是影响这一海域的最主要的水团.在丰度组成上,4月暖温带近海种占总丰度的98.79％,7月暖温带近海种也是组成丰度的重要部分,10月则是亚热带近海种丰度最高.丰度组成所反映的规律与种类数组成规律一致.上升流的存在导致夏季近海暖温带种大量出现,是影响这一海域浮游动物生态类群组成的重要因素;受长江径流和椒江径流的影响,近海种成为主要生态类群,是这一海域浮游动物的一个重要的生态特征.     

胃息肉与幽门螺杆菌感染关系研究

目的探讨胃息肉与幽门螺杆菌（Helicobacter pylori,H.pylori）感染关系。方法对1218例胃息肉同时进行H．pylori检查患者进行回顾性分析,分析胃息肉患者H.pylori感染率、胃息肉部位与H.pylori感染关系、胃息肉病理类型与H.pylori感染关系。结果发现胃息肉Hpylori感染患者532例,Hpylori感染率为43．7％。男性胃息肉患者H.pylori感染率为47．5％（216／455）,女性Hpriori感染率感染率为41．4％（316／763）（P〉0．05）,年龄〈20岁、20～39岁、40—59岁和≥60岁胃息肉H.priori感染率分别为41．7％、44．7％、41．6％和47．2％（P〉0．05）;胃窦胃角息肉H.pylori感染率高于其他部位（胃体、胃底和贲门）（P〈0．05）;炎性和增生性胃息肉H.priori感染率高于胃底腺和腺瘤性息肉（P〈0．05）。结论H.pylori感染可能与部分胃息肉发生有一定关系,需要进一步深入研究胃息肉的发生机制。     

光黑壳属植物内生菌的一个新纪录种

从采自河南境内伏牛山的野生碎米桠（Isodon rubescens （Lamiaceae））中分离到1株内生真菌,经鉴定为光黑壳属的中国新纪录种－Preussia dakotensis,,根据研究菌株对其进行了详细的形态描述、分子鉴定及分析讨论。